Sequencing:Article Title: Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers.
Article Snippet: Immunized by VACSERA Biological Products and Vaccines Holding Company, Egypt N/A Rabbit polyclonal anti-EDNRA – C-terminal (388-427) This work. .. N/A Rabbit polyclonal anti-Gnb antibodies against a conserved sequence of Gnb1, (C)-EGNVRVSRELAGHTGY AbdAlla et al., 2004 N/A Rabbit polyclonal anti-Gng antibodies against a conserved sequence of Gng2, SENPFREKKFFC AbdAlla et al., 2004 N/A Peroxidase-conjugated AffiniPure F(ab)2 Fragment Goat anti-Rabbit IgG, Fcg Fragment Specific (minimal cross-reaction to Human Serum Proteins) Jackson ImmunoResearch Laboratories Cat. No. 111-036-046; RRID: AB_2337944 Peroxidase-conjugated AffiniPure F(ab)2 Fragment goat anti-Mouse IgG, Fcg Fragment Specific (minimal crossreaction to Human, Bovine, and Horse Proteins) Jackson ImmunoResearch Laboratories Cat. No. 115-036-071; RRID: AB_2338524 Peroxidase-conjugated AffiniPure Sheep anti-mouse IgG, Fcg fragment-specific Jackson ImmunoResearch Laboratories Cat. No. 515-035-071; RRID: AB_2340297 MonoRab Rabbit anti-camelid VHH antibody [HRP] GenScript Cat. No. A01861 Bacterial and Virus Strains One Shot Stbl3TM Chemically Competent E. coli Invitrogen Cat. No. C7373-03 Biological Samples Placental biopsy specimens of maternal basal plate MRC (Medical Research Center), Faculty of Medicine, Cairo, Egypt Ain Shams University Hospitals, Cairo, Egypt Chemicals, Peptides, and Recombinant Proteins FITC (Fluorescein isothiocyanate) Thermo Scientific Cat. No. 46425 TRITC (5/6-tetramethylrhodamine isothiocyanate) Thermo Scientific Cat. No. 46112 Protein A, Peroxidase Conjugate Calbiochem Merck Millipore Cat. No. 539253 Myo-[2-3H]-Inositol PerkinElmer NET1156001MC [2,3,-prolyl-3,4-3H(N)]Bradykinin PerkinElmer NET706 Sar1,[125I]Tyr4,Ile8-angiotensin II PerkinElmer NEX248 Sar1, Ile4, Ile8-Angiotensin II trifluoroacetate salt (SII) Bachem H-6252 Sar-Arg-Val-Tyr-Ile-His-Pro-D-Ala-OH (TRV027, TRV12027; Violin et al., 2010) GenScript TRV12027 Bradykinin acetate salt Bachem H-1970 (Continued on next page) Cell 10, 1–16.e1–e8, January 10, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Angiotensin II acetate salt Bachem H-1705 HOE140 Sigma-Aldrich H-157 Losartan potassium Sigma-Aldrich 61188 BAPTA-AM Sigma-Aldrich A1076 L-carnitine hydrochloride Sigma-Aldrich C0283 XF Base Medium Seahorse Bioscience PN: 102353-100 Lipofactamine 2000 Invitrogen Cat. No. 116680-19 Protease Inhibitor Cocktail Sigma-Aldrich Cat. No. P8340 Protein phosphatase inhibitor cocktail Sigma-Aldrich Cat. No. PPC1010 Endoproteinase Glu-C (V8 protease) Sigma-Aldrich (Roche) Cat. No. 11 047 817 001 LR ClonaseTM II enzyme mix Invitrogen Cat. No. 11791100 Aqua-Polymount Polysciences Cat. No. 1860620 Polymount Xylene Polysciences Cat. No. 24176120 Protein G-Sepharose GE Healthcare (Sigma-Aldrich) Cat. No. P3296 AG 1-X8, mesh 100-200; BioRad) AG 1-X8, mesh 100-200; BioRad) AG 1-X8, mesh 100-200; BioRad) Amicon Ultra-15 PLBC Ultracel-PL membrane 3 kDa Merck Millipore (Sigma-Aldrich Cat. No. Z740199 Critical Commercial Assays Mouse VEGF R1/Flt-1 Quantikine ELISA kit R&D Systems Cat. No. MVR100 Human VEGF R1/Flt-1 Quantikine ELISA kit R&D Systems Cat. No. DVR100 Endothelin-1 Quantikine ELISA kit R&D Systems Cat. No. DET100 BCG Albumin Assay Kit Sigma-Aldrich Cat. No. MAK124 Creatinine Assay Kit Sigma-Aldrich Cat. No. MAK080 ACE kinetic Bühlmann Laboratories AG KK-ACK SensoLYTE 520 Mouse Renin Assay Kit AnaSPEC AS-72161 Duolink In Situ Probemaker PLUS Sigma-Aldrich DUO92009 Duolink In Situ Probemaker MINUS Sigma-Aldrich DUO92010 Duolink In Situ Detection Reagents Red Sigma-Aldrich DUO92008 RNeasy Mini k QIAGEN Cat. No. 74104 EndoFree Plasmid Maxi Kit QIAGEN Cat. No. 12362 LightCycler 480 SYBR Green I Master Roche Molecular Systems Cat. No. 04707516001 DAB Enhanced Liquid Substrate System Sigma-Aldrich Cat. No. D3939 ECL Prime Western Blotting Detection Reagent GE Healthcare Life Sciences Cat. No. RPN2232 Pierce ECL Plus Western Blotting Substrate Thermo Fisher Scientific Cat. No. 32132 Experimental Models: Cell Lines HEK293-AGTR1 Abd Alla et al., 2010 N/A HEK293-BDKRB2 Abd Alla et al., 2010 N/A
Virus:Article Title: Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers.
Article Snippet: Immunized by VACSERA Biological Products and Vaccines Holding Company, Egypt N/A Rabbit polyclonal anti-EDNRA – C-terminal (388-427) This work. .. N/A Rabbit polyclonal anti-Gnb antibodies against a conserved sequence of Gnb1, (C)-EGNVRVSRELAGHTGY AbdAlla et al., 2004 N/A Rabbit polyclonal anti-Gng antibodies against a conserved sequence of Gng2, SENPFREKKFFC AbdAlla et al., 2004 N/A Peroxidase-conjugated AffiniPure F(ab)2 Fragment Goat anti-Rabbit IgG, Fcg Fragment Specific (minimal cross-reaction to Human Serum Proteins) Jackson ImmunoResearch Laboratories Cat. No. 111-036-046; RRID: AB_2337944 Peroxidase-conjugated AffiniPure F(ab)2 Fragment goat anti-Mouse IgG, Fcg Fragment Specific (minimal crossreaction to Human, Bovine, and Horse Proteins) Jackson ImmunoResearch Laboratories Cat. No. 115-036-071; RRID: AB_2338524 Peroxidase-conjugated AffiniPure Sheep anti-mouse IgG, Fcg fragment-specific Jackson ImmunoResearch Laboratories Cat. No. 515-035-071; RRID: AB_2340297 MonoRab Rabbit anti-camelid VHH antibody [HRP] GenScript Cat. No. A01861 Bacterial and Virus Strains One Shot Stbl3TM Chemically Competent E. coli Invitrogen Cat. No. C7373-03 Biological Samples Placental biopsy specimens of maternal basal plate MRC (Medical Research Center), Faculty of Medicine, Cairo, Egypt Ain Shams University Hospitals, Cairo, Egypt Chemicals, Peptides, and Recombinant Proteins FITC (Fluorescein isothiocyanate) Thermo Scientific Cat. No. 46425 TRITC (5/6-tetramethylrhodamine isothiocyanate) Thermo Scientific Cat. No. 46112 Protein A, Peroxidase Conjugate Calbiochem Merck Millipore Cat. No. 539253 Myo-[2-3H]-Inositol PerkinElmer NET1156001MC [2,3,-prolyl-3,4-3H(N)]Bradykinin PerkinElmer NET706 Sar1,[125I]Tyr4,Ile8-angiotensin II PerkinElmer NEX248 Sar1, Ile4, Ile8-Angiotensin II trifluoroacetate salt (SII) Bachem H-6252 Sar-Arg-Val-Tyr-Ile-His-Pro-D-Ala-OH (TRV027, TRV12027; Violin et al., 2010) GenScript TRV12027 Bradykinin acetate salt Bachem H-1970 (Continued on next page) Cell 10, 1–16.e1–e8, January 10, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Angiotensin II acetate salt Bachem H-1705 HOE140 Sigma-Aldrich H-157 Losartan potassium Sigma-Aldrich 61188 BAPTA-AM Sigma-Aldrich A1076 L-carnitine hydrochloride Sigma-Aldrich C0283 XF Base Medium Seahorse Bioscience PN: 102353-100 Lipofactamine 2000 Invitrogen Cat. No. 116680-19 Protease Inhibitor Cocktail Sigma-Aldrich Cat. No. P8340 Protein phosphatase inhibitor cocktail Sigma-Aldrich Cat. No. PPC1010 Endoproteinase Glu-C (V8 protease) Sigma-Aldrich (Roche) Cat. No. 11 047 817 001 LR ClonaseTM II enzyme mix Invitrogen Cat. No. 11791100 Aqua-Polymount Polysciences Cat. No. 1860620 Polymount Xylene Polysciences Cat. No. 24176120 Protein G-Sepharose GE Healthcare (Sigma-Aldrich) Cat. No. P3296 AG 1-X8, mesh 100-200; BioRad) AG 1-X8, mesh 100-200; BioRad) AG 1-X8, mesh 100-200; BioRad) Amicon Ultra-15 PLBC Ultracel-PL membrane 3 kDa Merck Millipore (Sigma-Aldrich Cat. No. Z740199 Critical Commercial Assays Mouse VEGF R1/Flt-1 Quantikine ELISA kit R&D Systems Cat. No. MVR100 Human VEGF R1/Flt-1 Quantikine ELISA kit R&D Systems Cat. No. DVR100 Endothelin-1 Quantikine ELISA kit R&D Systems Cat. No. DET100 BCG Albumin Assay Kit Sigma-Aldrich Cat. No. MAK124 Creatinine Assay Kit Sigma-Aldrich Cat. No. MAK080 ACE kinetic Bühlmann Laboratories AG KK-ACK SensoLYTE 520 Mouse Renin Assay Kit AnaSPEC AS-72161 Duolink In Situ Probemaker PLUS Sigma-Aldrich DUO92009 Duolink In Situ Probemaker MINUS Sigma-Aldrich DUO92010 Duolink In Situ Detection Reagents Red Sigma-Aldrich DUO92008 RNeasy Mini k QIAGEN Cat. No. 74104 EndoFree Plasmid Maxi Kit QIAGEN Cat. No. 12362 LightCycler 480 SYBR Green I Master Roche Molecular Systems Cat. No. 04707516001 DAB Enhanced Liquid Substrate System Sigma-Aldrich Cat. No. D3939 ECL Prime Western Blotting Detection Reagent GE Healthcare Life Sciences Cat. No. RPN2232 Pierce ECL Plus Western Blotting Substrate Thermo Fisher Scientific Cat. No. 32132 Experimental Models: Cell Lines HEK293-AGTR1 Abd Alla et al., 2010 N/A HEK293-BDKRB2 Abd Alla et al., 2010 N/A
Recombinant:Article Title: Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers.
Article Snippet: Immunized by VACSERA Biological Products and Vaccines Holding Company, Egypt N/A Rabbit polyclonal anti-EDNRA – C-terminal (388-427) This work. .. N/A Rabbit polyclonal anti-Gnb antibodies against a conserved sequence of Gnb1, (C)-EGNVRVSRELAGHTGY AbdAlla et al., 2004 N/A Rabbit polyclonal anti-Gng antibodies against a conserved sequence of Gng2, SENPFREKKFFC AbdAlla et al., 2004 N/A Peroxidase-conjugated AffiniPure F(ab)2 Fragment Goat anti-Rabbit IgG, Fcg Fragment Specific (minimal cross-reaction to Human Serum Proteins) Jackson ImmunoResearch Laboratories Cat. No. 111-036-046; RRID: AB_2337944 Peroxidase-conjugated AffiniPure F(ab)2 Fragment goat anti-Mouse IgG, Fcg Fragment Specific (minimal crossreaction to Human, Bovine, and Horse Proteins) Jackson ImmunoResearch Laboratories Cat. No. 115-036-071; RRID: AB_2338524 Peroxidase-conjugated AffiniPure Sheep anti-mouse IgG, Fcg fragment-specific Jackson ImmunoResearch Laboratories Cat. No. 515-035-071; RRID: AB_2340297 MonoRab Rabbit anti-camelid VHH antibody [HRP] GenScript Cat. No. A01861 Bacterial and Virus Strains One Shot Stbl3TM Chemically Competent E. coli Invitrogen Cat. No. C7373-03 Biological Samples Placental biopsy specimens of maternal basal plate MRC (Medical Research Center), Faculty of Medicine, Cairo, Egypt Ain Shams University Hospitals, Cairo, Egypt Chemicals, Peptides, and Recombinant Proteins FITC (Fluorescein isothiocyanate) Thermo Scientific Cat. No. 46425 TRITC (5/6-tetramethylrhodamine isothiocyanate) Thermo Scientific Cat. No. 46112 Protein A, Peroxidase Conjugate Calbiochem Merck Millipore Cat. No. 539253 Myo-[2-3H]-Inositol PerkinElmer NET1156001MC [2,3,-prolyl-3,4-3H(N)]Bradykinin PerkinElmer NET706 Sar1,[125I]Tyr4,Ile8-angiotensin II PerkinElmer NEX248 Sar1, Ile4, Ile8-Angiotensin II trifluoroacetate salt (SII) Bachem H-6252 Sar-Arg-Val-Tyr-Ile-His-Pro-D-Ala-OH (TRV027, TRV12027; Violin et al., 2010) GenScript TRV12027 Bradykinin acetate salt Bachem H-1970 (Continued on next page) Cell 10, 1–16.e1–e8, January 10, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Angiotensin II acetate salt Bachem H-1705 HOE140 Sigma-Aldrich H-157 Losartan potassium Sigma-Aldrich 61188 BAPTA-AM Sigma-Aldrich A1076 L-carnitine hydrochloride Sigma-Aldrich C0283 XF Base Medium Seahorse Bioscience PN: 102353-100 Lipofactamine 2000 Invitrogen Cat. No. 116680-19 Protease Inhibitor Cocktail Sigma-Aldrich Cat. No. P8340 Protein phosphatase inhibitor cocktail Sigma-Aldrich Cat. No. PPC1010 Endoproteinase Glu-C (V8 protease) Sigma-Aldrich (Roche) Cat. No. 11 047 817 001 LR ClonaseTM II enzyme mix Invitrogen Cat. No. 11791100 Aqua-Polymount Polysciences Cat. No. 1860620 Polymount Xylene Polysciences Cat. No. 24176120 Protein G-Sepharose GE Healthcare (Sigma-Aldrich) Cat. No. P3296 AG 1-X8, mesh 100-200; BioRad) AG 1-X8, mesh 100-200; BioRad) AG 1-X8, mesh 100-200; BioRad) Amicon Ultra-15 PLBC Ultracel-PL membrane 3 kDa Merck Millipore (Sigma-Aldrich Cat. No. Z740199 Critical Commercial Assays Mouse VEGF R1/Flt-1 Quantikine ELISA kit R&D Systems Cat. No. MVR100 Human VEGF R1/Flt-1 Quantikine ELISA kit R&D Systems Cat. No. DVR100 Endothelin-1 Quantikine ELISA kit R&D Systems Cat. No. DET100 BCG Albumin Assay Kit Sigma-Aldrich Cat. No. MAK124 Creatinine Assay Kit Sigma-Aldrich Cat. No. MAK080 ACE kinetic Bühlmann Laboratories AG KK-ACK SensoLYTE 520 Mouse Renin Assay Kit AnaSPEC AS-72161 Duolink In Situ Probemaker PLUS Sigma-Aldrich DUO92009 Duolink In Situ Probemaker MINUS Sigma-Aldrich DUO92010 Duolink In Situ Detection Reagents Red Sigma-Aldrich DUO92008 RNeasy Mini k QIAGEN Cat. No. 74104 EndoFree Plasmid Maxi Kit QIAGEN Cat. No. 12362 LightCycler 480 SYBR Green I Master Roche Molecular Systems Cat. No. 04707516001 DAB Enhanced Liquid Substrate System Sigma-Aldrich Cat. No. D3939 ECL Prime Western Blotting Detection Reagent GE Healthcare Life Sciences Cat. No. RPN2232 Pierce ECL Plus Western Blotting Substrate Thermo Fisher Scientific Cat. No. 32132 Experimental Models: Cell Lines HEK293-AGTR1 Abd Alla et al., 2010 N/A HEK293-BDKRB2 Abd Alla et al., 2010 N/A
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